9 C.F.R. § 113.32 Detection of Brucella contamination.
Title 9 - Animals and Animal Products
The test for detection of Brucella contamination provided in this section shall be conducted when such a test is prescribed in an applicable Standard Requirement or in a filed Outline of Production for the product. (a) One ml of the minced tissue used as the source of cells or 1 ml of the extract of the tissue prior to the addition of antibiotics, diluent and stabilizer, shall be inoculated onto each of three tryptose agar plates and incubated in a 10 percent CO2 atmosphere at a temperature of 35–37 °C for at least 7 days. (b) If colonies are identified as Brucella, the biological product is unsatisfactory. (c) If colonies suspicious of Brucella are observed but cannot be identified as a Brucella species, either (1) The biological product shall be regarded as unsatisfactory and destroyed; or (2) Further subculture or other procedures shall be carried out until a positive identification can be made. [38 FR 29888, Oct. 30, 1973]
Title 9: Animals and Animal Products
PART 113—STANDARD REQUIREMENTS
Standard Procedures
§ 113.32 Detection of Brucella contamination.
