14 C.F.R. § 121.555 Compliance with approved routes and limitations: Domestic and flag operations.
Title 14 - Aeronautics and Space
No pilot may operate an airplane in scheduled air transportation— (a) Over any route or route segment unless it is specified in the certificate holder's operations specifications; or (b) Other than in accordance with the limitations in the operations specifications. [Doc. No. 6258, 29 FR 19219, Dec. 31, 1964, as amended by Amdt. 121–253, 61 FR 2614, Jan. 26, 1996]
Title 14: Aeronautics and Space
PART 121—OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS
Subpart T—Flight Operations
§ 121.555 Compliance with approved routes and limitations: Domestic and flag operations.

