27 C.F.R. § 21.98 Bone oil (Dipple's oil).
Title 27 - Alcohol, Tobacco Products and Firearms
(a) Color. The color shall be a deep brown. (b) Distillation range. When 100 ml are distilled in the manner described for pyridine bases, not more than 5.0 ml should distill below 90 °C. (c) Pyrrol reaction. Prepare a 1.0 percent solution of bone oil in 95 percent alcohol. Prepare a second solution containing 0.025 percent bone oil by diluting 2.50 ml of the first solution to 100 ml with 95 percent alcohol. Dip a splinter of pine, previously moistened with concentrated hydrochloric acid, into 10 ml of the 0.025 percent bone oil solution. After a few minutes the splinter should show a distinct red coloration. (d) Reaction with mercuric chloride. Add 5 ml of the 1.0 percent bone oil solution above to 5 ml of a 2 percent alcoholic solution of mercuric chloride. A turbidity is formed at once which separates into a flocculent precipitate on standing several minutes. Add 5.0 ml of the 0.025 percent bone oil solution to 5.0 ml of a 2.0 percent alcoholic solution of mercuric chloride. A faint turbidity appears after several minutes. [T.D. ATF–133, 48 FR 24673, June 2, 1983. Redesignated by T.D. ATF–442, 66 FR 12854, Mar. 1, 2001]
Title 27: Alcohol, Tobacco and Firearms
PART 21—FORMULAS FOR DENATURED ALCOHOL AND RUM
Subpart E—Specifications for Denaturants
§ 21.98 Bone oil (Dipple's oil).
