9 C.F.R. § 147.13 Procedure for bacteriological culturing of eggshells for colon bacilli organisms.
Title 9 - Animals and Animal Products
Proper precautions to avoid environmental contamination of the samples during the collection and laboratory process, and proper handling of the samples following collection are essential. Each State Inspector involved in eggshell culture activities must receive instruction in the necessary sanitation procedures, sampling procedures, and sample handling by the authorized laboratory involved. The Official State Agency will maintain a record showing that the required instruction was given to each State Inspector. (a) Sample selection. Forty (40) eggs in the top flats of each of three randomly selected cases of sanitized eggs from each flock will be utilized for each sampling. (b) Swab procedure. A 2.5 centimeter diameter circular area of the large end of each of the eggs will be rubbed with a sterile swab previously moistened with sterile lactose broth, or other suitable liquid media provided by the authorized laboratory. One swab will be used for five eggs, and four swabs will be pooled to each sterile, capped tube provided by the authorized laboratory. (1) From the tube containing four swabs and lactose broth or other suitable media, 1 ml. will be transferred to 10 ml. lactose in a fermentation tube. (2) Incubate at 37 °C for 48 hours. The presence of acid, and gas in the amount of 10 percent or more after 24 and 48 hours of incubation, provides a presumptive conclusion of the presence of colon bacilli organisms. [41 FR 14256, Apr. 2, 1976. Redesignated at 44 FR 61586, Oct. 26, 1979, and amended at 59 FR 12805, Mar. 18, 1994]
Title 9: Animals and Animal Products
PART 147—AUXILIARY PROVISIONS ON NATIONAL POULTRY IMPROVEMENT PLAN
Subpart B—Bacteriological Examination Procedure
§ 147.13 Procedure for bacteriological culturing of eggshells for colon bacilli organisms.
